Identification of Soil-Transmitted Helminth (STH) on Selected Vegetables in a Public Market
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Abstract
This study aimed to identify the Soil-Transmitted Helminth (STH) on selected
vegetable samples in a public market. The study was conducted between January
2021 to March 2021. A total of 165 vegetable samples were collected and grouped
according to its type. Root crops consisted of carrots, radish, and sweet potatoes
while leafy crops include cabbage, pechay, and lettuce. For the fruit crops,
cucumbers, eggplant, and tomatoes, and lastly, bulb crops are garlic and spring
onion. The vegetable samples were washed using normal saline and distilled water
then examined by sedimentation technique, a process by letting particles suspended
in water settle out of the suspension under gravity. The mixture of 10000?mL of
physiological saline solution (0.9% sodium chloride) and 10000 mL distilled water
was divided into 11 equal portions and used to rinse each type of vegetable samples.
Then, the washing water was collected and left overnight to allow sedimentation then centrifuged at 2000 rpm for 15?minutes to separate the parasites from the washing
water. The researchers prepared two slides per each sediment and examined it
under the microscope using 10x, 40x, and 100x magnification. The soil-transmitted
helminth found in the vegetable samples were Hookworms, Strongyloides stercoralis,
Giardia spp., Ascaris lumbricoides, Entamoeba histolytica, Tapeworm, and
Enterobius vermicularis. Cabbage is the most contaminated vegetable while eggplant
is the least. Since vegetables are considered a good source of parasites in the study
area, proper washing of vegetables before selling is significantly associated with
decreased parasitic contamination.
Keywords
Soil-Transmitted Helminth (STH)
Strongyloides stercoralis
Giardia spp.
Ascaris lumbricoides
Entamoeba histolytica
Tapeworm
Enterobius vermicularis
public market
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APPLIED TECHNOLOGY, SCIENCE , INDUSTRYBUSINESS, ECONOMICS AND INDUSTRY 4.0 RESEARCHBUSINESS, INDUSTRY, LIVELIHOOD and FOOD SECURITYCOMMUNITY DEVELOPMENT and SOCIAL SUSTAINABILITYEDUCATION 4.0 AND WORKFORCE 4.0 RESEARCHEDUCATION and EDUCATION MANAGEMENTENVIRONMENTAL CONSERVATION, PROTECTION and DEVELOPMENTENVIRONMENTAL PROTECTION, DEVELOPMENT, AND CONSERVATION RESEARCHHEALTH and WELLNESS PROGRAM DEVELOPMENTHEALTH RESEARCH, DEVELOPMENT, INNOVATION AND EXTENSIONHUMANITIES, ARTS, CULTURE and TOURISMLEGAL, LAW ENFORCEMENT AND CRIMINOLOGY RESEARCHPOLITICS, SOCIETY, AND CULTURE RESEARCHTECHNOLOGY, ENGINEERING, AND INDUSTRY 4.0 RESEARCH